Dictionary TreatmentIn Vitro Fertilization (IVF)Progesterone In Oil Progesterone In Oil Reviewed by Dr. Temeka Zore OB/GYN, REI View our Editorial Policy Published: August 5, 2017 What Does Progesterone In Oil Mean? Progesterone in oil (PIO) is an injectable form of the hormone progesterone that is sometimes used as part of the process of in vitro fertilization (IVF) as a woman is preparing for an embryo transfer. Advertisement FertilitySmarts Explains Progesterone In Oil Progesterone is a primary pregnancy hormone that helps to prepare a plush inner lining of the uterus required for implantation of an embryo. PIO is an injectable form of progesterone combined with oil to allow the body to absorb it easier. Injections vs. Suppositories PIO formulation raises the serum progesterone concentrations at a relatively higher rate compared to the vaginal route which usually yields lower serum levels of progesterone but higher progesterone concentrations in the inner lining of the uterus. Because PIO injections are very viscose, a larger gauge needle is used which can be painful, and can cause bruising and skin irritation. The type of progesterone used by your reproductive endocrinologist may vary depending on whether you are having a fresh or frozen embryo transfer and whether you are doing a natural or synthetic cycle. PIO Shot Steps PIO can either be injected by the patient themselves or by a helper, which may be easier. The steps of using a PIO vial often look like: Follow all directions as provided by your doctor First, wash your hands thoroughly Draw up the progesterone from the vial into the syringe, recap it, and place it in warm water to make the contents thinner Choose the site to inject the shot, typically the upper, outer quadrant of the buttocks Cleanse the area with an alcohol swab Aspirate quickly and then insert the needle into the skin Withdraw the needle immediately and press the injection site with a spirit swab for a few seconds A warm compress may help with any discomfort Other than the side effects mentioned above, PIO injections can cause headaches, nausea, increased body/facial hair, and weight gain/loss. Advertisement Synonyms Progesterone Shot Progesterone In Oil Shot Progesterone Injection Progesterone In Oil Injection Share This Term> Related Terms Single Embryo Transfer Trilaminar Endometrium Related Reading 14 Onesies that Celebrate IVF Babies IVF Pregnancy Announcements Fertility Podcasts Relatable Words After a Miscarriage Let’s Stop Arguing About Whether or Not Stress Causes Infertility Books About Infertility Tags Treatment Menstrual Cycle Miscarriage Hormones In Vitro Fertilization (IVF) Difficulty Uterus Trending Articles Pregnancy IVF Pregnancy Announcements Egg Retrieval From Eggs to Blastocysts: Understanding IVF Attrition Cervical Mucus Identifying Stages of Cervical Mucus Artificial Insemination (AI) Implantation Calendar: What is Happening During the Two Week Wait?